applied 433 peptide synthesiser Search Results


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model 433 peptide synthesizer  (Thermo Fisher)
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synthesiser  (Thermo Fisher)
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applied 433 polypeptide synthesizer  (Thermo Fisher)
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Thermo Fisher applied 433 polypeptide synthesizer
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cyclin b1  (Santa Cruz Biotechnology)
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Biochemical analysis of synchronized human iPS (A6 and D1), hES (H9) and/or fibroblasts cells (TIG1) corroborate the finding that human iPS cells have an abbreviated G1 phase. (A) The relative RNA expression of cell cycle indicators; histone H4, <t>Cyclin</t> E2, Cyclin B2, HINFP and p220NPAT was analyzed using mRNA isolated from actively proliferating and nocodazole synchronized human iPS (asynchronous [Asy], 0h [G2/M], 2h [G1] and 4h [early S] after release) and hES cells (asynchronous [Asy], 0h [G2/M], 1.5h [G1] and 4h [early S] after release). (B) For comparison, the relative RNA expression of some of the cell cycle indicators; histone H4, Cyclin E2, Cyclin B2, HINFP and p220NPAT was analyzed in actively proliferating and nocodazole synchronized human normal fibroblasts TIG1 (asynchronous [Asy], 0h [mitosis-M], 6h [G1] and 16h [S] after release). (C) Protein expression analysis of human iPS (A6 and D1) and hES (H9) for cell cycle indicators (Cyclin E2, Cyclin B2 and HINFP) also reveals rapid entry into S-phase. (D) FACS analysis of synchronized human iPS (A6) and hES (H9) cells shows that within 2–3 h after exit from mitosis cells enter S-phase. Statistical analysis is done against/compared to G2/M (Figure 3A) or M (figure 3B), except if noted otherwise (brackets). †, approaching significance (0.05<P<0.1); *, P<0.05; **, P<0.01.
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433 synthesizer  (Thermo Fisher)
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Thermo Fisher 433 synthesizer
Biochemical analysis of synchronized human iPS (A6 and D1), hES (H9) and/or fibroblasts cells (TIG1) corroborate the finding that human iPS cells have an abbreviated G1 phase. (A) The relative RNA expression of cell cycle indicators; histone H4, <t>Cyclin</t> E2, Cyclin B2, HINFP and p220NPAT was analyzed using mRNA isolated from actively proliferating and nocodazole synchronized human iPS (asynchronous [Asy], 0h [G2/M], 2h [G1] and 4h [early S] after release) and hES cells (asynchronous [Asy], 0h [G2/M], 1.5h [G1] and 4h [early S] after release). (B) For comparison, the relative RNA expression of some of the cell cycle indicators; histone H4, Cyclin E2, Cyclin B2, HINFP and p220NPAT was analyzed in actively proliferating and nocodazole synchronized human normal fibroblasts TIG1 (asynchronous [Asy], 0h [mitosis-M], 6h [G1] and 16h [S] after release). (C) Protein expression analysis of human iPS (A6 and D1) and hES (H9) for cell cycle indicators (Cyclin E2, Cyclin B2 and HINFP) also reveals rapid entry into S-phase. (D) FACS analysis of synchronized human iPS (A6) and hES (H9) cells shows that within 2–3 h after exit from mitosis cells enter S-phase. Statistical analysis is done against/compared to G2/M (Figure 3A) or M (figure 3B), except if noted otherwise (brackets). †, approaching significance (0.05<P<0.1); *, P<0.05; **, P<0.01.
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433 peptide synthesizer  (Thermo Fisher)
90
Thermo Fisher 433 peptide synthesizer
Biochemical analysis of synchronized human iPS (A6 and D1), hES (H9) and/or fibroblasts cells (TIG1) corroborate the finding that human iPS cells have an abbreviated G1 phase. (A) The relative RNA expression of cell cycle indicators; histone H4, <t>Cyclin</t> E2, Cyclin B2, HINFP and p220NPAT was analyzed using mRNA isolated from actively proliferating and nocodazole synchronized human iPS (asynchronous [Asy], 0h [G2/M], 2h [G1] and 4h [early S] after release) and hES cells (asynchronous [Asy], 0h [G2/M], 1.5h [G1] and 4h [early S] after release). (B) For comparison, the relative RNA expression of some of the cell cycle indicators; histone H4, Cyclin E2, Cyclin B2, HINFP and p220NPAT was analyzed in actively proliferating and nocodazole synchronized human normal fibroblasts TIG1 (asynchronous [Asy], 0h [mitosis-M], 6h [G1] and 16h [S] after release). (C) Protein expression analysis of human iPS (A6 and D1) and hES (H9) for cell cycle indicators (Cyclin E2, Cyclin B2 and HINFP) also reveals rapid entry into S-phase. (D) FACS analysis of synchronized human iPS (A6) and hES (H9) cells shows that within 2–3 h after exit from mitosis cells enter S-phase. Statistical analysis is done against/compared to G2/M (Figure 3A) or M (figure 3B), except if noted otherwise (brackets). †, approaching significance (0.05<P<0.1); *, P<0.05; **, P<0.01.
433 Peptide Synthesizer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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applied 433 syntesizer  (Thermo Fisher)
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Thermo Fisher applied 433 syntesizer
Biochemical analysis of synchronized human iPS (A6 and D1), hES (H9) and/or fibroblasts cells (TIG1) corroborate the finding that human iPS cells have an abbreviated G1 phase. (A) The relative RNA expression of cell cycle indicators; histone H4, <t>Cyclin</t> E2, Cyclin B2, HINFP and p220NPAT was analyzed using mRNA isolated from actively proliferating and nocodazole synchronized human iPS (asynchronous [Asy], 0h [G2/M], 2h [G1] and 4h [early S] after release) and hES cells (asynchronous [Asy], 0h [G2/M], 1.5h [G1] and 4h [early S] after release). (B) For comparison, the relative RNA expression of some of the cell cycle indicators; histone H4, Cyclin E2, Cyclin B2, HINFP and p220NPAT was analyzed in actively proliferating and nocodazole synchronized human normal fibroblasts TIG1 (asynchronous [Asy], 0h [mitosis-M], 6h [G1] and 16h [S] after release). (C) Protein expression analysis of human iPS (A6 and D1) and hES (H9) for cell cycle indicators (Cyclin E2, Cyclin B2 and HINFP) also reveals rapid entry into S-phase. (D) FACS analysis of synchronized human iPS (A6) and hES (H9) cells shows that within 2–3 h after exit from mitosis cells enter S-phase. Statistical analysis is done against/compared to G2/M (Figure 3A) or M (figure 3B), except if noted otherwise (brackets). †, approaching significance (0.05<P<0.1); *, P<0.05; **, P<0.01.
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revertaid first strand cdna synthesis kit  (Thermo Fisher)
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Biochemical analysis of synchronized human iPS (A6 and D1), hES (H9) and/or fibroblasts cells (TIG1) corroborate the finding that human iPS cells have an abbreviated G1 phase. (A) The relative RNA expression of cell cycle indicators; histone H4, <t>Cyclin</t> E2, Cyclin B2, HINFP and p220NPAT was analyzed using mRNA isolated from actively proliferating and nocodazole synchronized human iPS (asynchronous [Asy], 0h [G2/M], 2h [G1] and 4h [early S] after release) and hES cells (asynchronous [Asy], 0h [G2/M], 1.5h [G1] and 4h [early S] after release). (B) For comparison, the relative RNA expression of some of the cell cycle indicators; histone H4, Cyclin E2, Cyclin B2, HINFP and p220NPAT was analyzed in actively proliferating and nocodazole synchronized human normal fibroblasts TIG1 (asynchronous [Asy], 0h [mitosis-M], 6h [G1] and 16h [S] after release). (C) Protein expression analysis of human iPS (A6 and D1) and hES (H9) for cell cycle indicators (Cyclin E2, Cyclin B2 and HINFP) also reveals rapid entry into S-phase. (D) FACS analysis of synchronized human iPS (A6) and hES (H9) cells shows that within 2–3 h after exit from mitosis cells enter S-phase. Statistical analysis is done against/compared to G2/M (Figure 3A) or M (figure 3B), except if noted otherwise (brackets). †, approaching significance (0.05<P<0.1); *, P<0.05; **, P<0.01.
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Image Search Results


Biochemical analysis of synchronized human iPS (A6 and D1), hES (H9) and/or fibroblasts cells (TIG1) corroborate the finding that human iPS cells have an abbreviated G1 phase. (A) The relative RNA expression of cell cycle indicators; histone H4, Cyclin E2, Cyclin B2, HINFP and p220NPAT was analyzed using mRNA isolated from actively proliferating and nocodazole synchronized human iPS (asynchronous [Asy], 0h [G2/M], 2h [G1] and 4h [early S] after release) and hES cells (asynchronous [Asy], 0h [G2/M], 1.5h [G1] and 4h [early S] after release). (B) For comparison, the relative RNA expression of some of the cell cycle indicators; histone H4, Cyclin E2, Cyclin B2, HINFP and p220NPAT was analyzed in actively proliferating and nocodazole synchronized human normal fibroblasts TIG1 (asynchronous [Asy], 0h [mitosis-M], 6h [G1] and 16h [S] after release). (C) Protein expression analysis of human iPS (A6 and D1) and hES (H9) for cell cycle indicators (Cyclin E2, Cyclin B2 and HINFP) also reveals rapid entry into S-phase. (D) FACS analysis of synchronized human iPS (A6) and hES (H9) cells shows that within 2–3 h after exit from mitosis cells enter S-phase. Statistical analysis is done against/compared to G2/M (Figure 3A) or M (figure 3B), except if noted otherwise (brackets). †, approaching significance (0.05<P<0.1); *, P<0.05; **, P<0.01.

Journal:

Article Title: Reprogramming the pluripotent cell cycle: restoration of an abbreviated G1 phase in human induced pluripotent stem (iPS) cells

doi: 10.1002/jcp.22440

Figure Lengend Snippet: Biochemical analysis of synchronized human iPS (A6 and D1), hES (H9) and/or fibroblasts cells (TIG1) corroborate the finding that human iPS cells have an abbreviated G1 phase. (A) The relative RNA expression of cell cycle indicators; histone H4, Cyclin E2, Cyclin B2, HINFP and p220NPAT was analyzed using mRNA isolated from actively proliferating and nocodazole synchronized human iPS (asynchronous [Asy], 0h [G2/M], 2h [G1] and 4h [early S] after release) and hES cells (asynchronous [Asy], 0h [G2/M], 1.5h [G1] and 4h [early S] after release). (B) For comparison, the relative RNA expression of some of the cell cycle indicators; histone H4, Cyclin E2, Cyclin B2, HINFP and p220NPAT was analyzed in actively proliferating and nocodazole synchronized human normal fibroblasts TIG1 (asynchronous [Asy], 0h [mitosis-M], 6h [G1] and 16h [S] after release). (C) Protein expression analysis of human iPS (A6 and D1) and hES (H9) for cell cycle indicators (Cyclin E2, Cyclin B2 and HINFP) also reveals rapid entry into S-phase. (D) FACS analysis of synchronized human iPS (A6) and hES (H9) cells shows that within 2–3 h after exit from mitosis cells enter S-phase. Statistical analysis is done against/compared to G2/M (Figure 3A) or M (figure 3B), except if noted otherwise (brackets). †, approaching significance (0.05

Article Snippet: The following antibodies were used cyclin B1 (H-433), CDK2 (M2), (all from Santa Cruz Biotechnology), cyclin E (HE-12) (BD Bioscience, San Jose, CA) and HiNF-P ( Mitra et al, 2003 ). cDNA synthesis and real time quantitative PCR (qPCR) Total RNA was isolated using TRIzol from actively proliferating or mitotically synchronized and released human ES, iPS and TIG1 cells, subjected to DNase I digestion and purified by column chromatography (DNA-free RNA Kit, ZYMO Research, Orange, CA) or the Qiagen RNeasy Plus Mini Kit (Qiagen, Valencia, CA).

Techniques: RNA Expression, Isolation, Comparison, Expressing